Affiliation of Author(s):[1]Univ Elect Sci & Technol China, Sichuan Prov Peoples Hosp, Personalized Drug Therapy Key Lab Sichuan Prov, Sch Med,Dept Pharm, Chengdu 610072, Peoples R China;[2]China Univ Petr, Coll New Energy & Mat, Beijing Key Lab Biogas Upgrading Utilizat, State Key Lab Heavy Oil Proc, Beijing 102249, Peoples R China;[3]Sichuan Univ, West China Hosp, State Key Lab Biotherapy & Canc, Chengdu 610041, Peoples R China;[4]Collaborat Innovat Ctr, Chengdu 610041, Peoples R China
Journal:CHINESE CHEMICAL LETTERS
Key Words:Carbon dots; Fluorescent; Cell imaging; Erythrocyte; Blood compatibility
Abstract:Carbon dots have unique advantages in biological applications owing to their excellent optical properties. However, the biosafety evaluation of carbon dots has limitations owing to cytotoxicity in vitro, and there is little pre-safety evaluation before in vivo and clinical applications. Whether the carbon dots are or not suitable for applications in vivo, evaluation analysis can be made based on hemolysis and changes in erythrocyte morphology. In this work, a green fluorescent N, S-doped carbon dots (N, S-CDs) were obtained by hydrothermal method, tobias acid, and m-phenylenediamine as precursors. N, S-CDs not only possessed excellent dispersibility, uniform particle size, high quantum yield (37.2%) and stable photoluminescence property but also retain their photostability and strong fluorescence intensity in the acid/alkaline solutions, different ionic strengths (NaCl) and under 365 nm UV illumination. Moreover, the N, S-CDs displayed low cytotoxicity and high cellular uptake efficiency in human umbilical vein endothelial cells (HUVEC) and excellent blood compatibility to the erythrocyte. It is foreseeable that N, S-CDs could be further studied as a promising biological imaging agent in vivo. (C) 2020 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
Document Type:Article
Volume:31
Issue:3
Page Number:769-773
ISSN No.:1001-8417
Translation or Not:no