We are particularly interested in understanding the phylogenetic relationships among different groups of fungi, including pathogenic and saprobic, which are belong to the class Sordariomycetes. As a part of an ongoing study, we reviewed 107 families of this second largest class of ascomycetes and provided an updated outline and a backbone tree based on multi gene sequence data. However, a comprehensive phylogenetic classification frame for the whole class is still largely missing. Also, Sordariomycetes is under represented in terms of collections and sequence data and many genera are in need of taxonomic revision. Therefore, greater numbers of sampling and obtain sequence data from fresh collection are essential to stabilize the natural classification of the group. So, in order to achieve these goals we are extensively investigating fresh collections from freshwater and terrestrial niches including karst and bambusicolous habitats.