1. Highly dynamic and multiplxed live-cell imaging/多色动态活细胞光学成像

Synergistical effect of various organelles (organelle interactions) is increasingly recognized to have a vital role in executing diverse physiological cellular functions. Fluorescence microscopy, with target specificity and compatibility with live cells, is a valuable tool for studying complex biological systems and processes. We aim to develop highly multiplexed and high-speed spectral microscopy techniques to visualize intracellular activities and quantify chemical/physical parameters of cellular organelles at high spatiotemporal resolution.

在亚细胞结构层面，荧光光谱成像技术与具有多重态光谱特征的生物探针相结合，为活细胞内物化参数的高时空定量观测提供了丰富的可能性。我们致力于进一步扩展光谱成像的空间维度，发展具有多目标、多参数并行动态表征能力的三维成像工具。

See Publications: Light: Science & Applications 2021, 10, 97; Biomed. Opt. Exp. 2022, 11, 6048; Developmental Cell 2022, 57, 1; ACS Photonics 2024, 11, 3454

2. Single-molecule and Super-resolution microscopy/单分子超分辨光学显微成像

As a bottom-up approach, super-resolution microscopy through single-molecule localization achieves ~10 nm spatial resolution that challenges the mindset of traditional microscopy. By characterizing the multidimensional information of single-molecule signals, multifunctional super-resolution microscopy are developed for cell biology beyond the structural information.

在单分子层面，单分子荧光探测技术是一种普适性的单分子分析手段，我们将开发能够测定单分子多维信息的高通量荧光分析技术，构建一系列能够对活细胞内多维参数进行功能性超分辨表征的成像手段。

See Publications: Nature Methods 2020, 17, 524; J. Am. Chem. Soc. 2020, 142, 18866; Nano Letters 2023, 23, 1711; ACS Nano 2021, 15, 12483; J. Am. Chem. Soc. 2022, 144, 4839